The manuscript should include an abstract that is between 250 and 300 words long.

Checks for a list of contributing authors names and affiliations in the manuscript.

Checks if the role and contributions of all credited authors are stated within the manuscript. For example: "SD, W-YJL, and MC designed and completed the experimental work. WI provided samples and insights from the HCV Research UK Biobank. ADSF, EA-C, CB, and JM completed the sequencing work and analysis..." This statement is usually placed after the conclusion.

Checks if citations appear in the abstract. The abstract should focus on the original research, be self-contained and fully understandable without external references.

To comply with this check, authors should report any relevant financial or personal relationships that could be viewed as inappropriately influencing the work or hindering transparency. If there is no disclosure to be made, that should be clearly mentioned in the manuscript too. This statement is usually placed after the conclusion.

The corresponding authors email address should be included in the manuscript to let editors know how to get in touch. It is usually placed after the abstract along with other metadata.

Checks if ethics statements are included. To pass, authors should document the studys ethical compliance or declare that the manuscript does not report any studies involving human or animal participants. If the study involves human subjects, declare how written informed consent was obtained. If it includes case reports/case series, provide appropriate details. In case of animal subjects, declare whether the investigative methods conform to the standards of good research practice. The ethics statement is usually placed after the conclusion.

Checks if figure legends are included and appear consecutively within the manuscript.

Checks if figures and tables with legends are cited within the manuscript. Every visual element included in the manuscript should also be mentioned in the text.

Checks if funding statements are included. Authors should disclose all sources of funding, including grants, research programs and institutional support. For example: "The work was supported by the Wellcome Trust (grant no. 087792), and the Medical Research Council (grant nos. 0900278 and U117532067). The funders had no role in study design, data collection or analysis.” This statement is usually placed after the conclusion.

Checks if a list of keywords is provided. For example: "Keywords: Adoptive cellular immunotherapy, cancer, immunohistochemistry, prognosis." This is usually placed after the abstract along with other metadata.

Cite recent sources when possible. To pass this check, less than 10% of references should be 10+ years old.

Every reference should be cited in the main text of the manuscript to pass this check.

The manuscript should include a reference section with at least 5 and at most 100 sources.

Including a large number of self-citations in the manuscript is discouraged. This check fails if more than 15 self-citations are found or more than 80% of the references are self-citations.

Checks if table legends are included and appear consecutively within the manuscript.

The title should include the key concepts investigated and have a length of 5 to 25 words.

Covers issues with capitalization, hyphenation, punctuation, spacing etc. Counts as failed if there are more than 20 suggestions per 1,000 words.

Covers conciseness, redundancy, transition and flow, and suggests improved phrasing. Counts as failed if there are more than 10 suggestions per 1,000 words.

Covers issues with fluency, informal usage, and word choice. Counts as failed if there are more than 10 suggestions per 1,000 words.

Covers issues with article usage, conjunctions, determiners, preposition usage, spelling, and phrasing. Counts as failed if there are more than 60 suggestions per 1000 words.

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